What is the difference between a fixed-angle and a swing-out rotor? 

By Kim McDonald, 27 June 2023

What is the difference between a fixed-angle and a swing-out rotor? In a swing-out rotor, the buckets swing out horizontally, allowing for horizontal centrifugation and sample separation. Samples within a fixed-angle rotor remain at a fixed angle throughout separation, causing sedimentation to occur at an angle. There are pros and cons to each rotor type and applications best suited for each. 

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Percoll Gradient Centrifugation: What to consider when isolating cells, subcellular particles, and virus

By Kim McDonald, 22 January 2023

Percoll gradients can be used to isolate specific cells, subcellular particles, and viral particles via centrifugation based on their individual densities. Gradients can be pre-formed before the isopycnic separation of cells at low speeds. However, viruses and subcellular particles generally will not efficiently separate on pre-formed gradients. In this case, in situ gradient preparation is needed where the sample is diluted in the Percoll solution prior to centrifugation; particles are separated simultaneously to gradient formation.

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I have a cold room, why do I need a refrigerated centrifuge?

By Kim McDonald, 05 October 2022

Though precise temperature control may not be required for many applications, some analytes are temperature-sensitive and require stable temperature control.  Refrigerated centrifuges counteract the temperature fluctuations that occur during centrifugation, making them suitable for temperature-sensitive analytes. 

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What is the optimal centrifugation protocol for preparing PRP?

By Doran Amos, 17 August 2021

Platelet-rich plasma (PRP) is widely used to promote tissue repair and bone growth—but what is the best way to prepare it? In this blog, we guide you through the latest research and recommendations on optimizing the collection, centrifugation and storage of PRP...

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How to Make a Lab Centrifuge

By Aimee O'Driscoll, 04 July 2021

Want to create your own centrifuge? We review a range of "instructables" including models using paper and string, a salad spinner, and a Dremel tool.

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