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Eppendorf 5427 R Refrigerated Centrifuge

Category Micro Centrifuges
Manufacturer Eppendorf
Country of manufacture Germany

The refrigerated Eppendorf Centrifuge 5427 R was designed with high-throughput research applications in mind. With its aerosol-tight 48-place rotor it handles even the toughest challenges in a safe and reliable manner to provide you the efficiency boost you need. All aerosol-tight rotors are equipped with the Eppendorf QuickLock® lids for easy rotor lid opening and closing. Choose the Centrifuge 5427 R if a 24-place microcentrifuge doesn't meet your needs regarding tube capacity and if you don't need the extra versatility our cross-over Centrifuge 5430 R can offer you.


  • Rotor capacity: 48 × 1.5/2.0 mL, 12 × 5.0 mL; Eppendorf QuickLock® rotor allows for fast and ergonomic lid locking
  • Max. speed: 25,000 × g (16,220 rpm)
  • High capacity 48-place rotor significantly increases productivity to save you valuable time
  • Soft touch one-finger closure for ergonomic operation
  • Temperature range from -11 °C to 40 °C
  • FastTemp function for quick pre-cooling of the centrifuge, e.g., from ~ 23 °C to 4 °C in only 11 minutes
  • ECO shut-off can be programmed to engage after 1, 2, 4 or 8 hours of non-use for maximized energy savings and extended compressor life
  • Built-in condensation drain to eliminate water accumulation
  • Automatic rotor recognition and imbalance detection for maximized operational safety
    Key Attributes 
    RPM 16,220
    RCF 25,000 × g
    Refrigeration Yes
    Capacity 48 × 1.5/2.0 mL
    Warranty (years) 2
    Manufacturer Specifications
    Model 5427 R
    Max. RCF 25,000 × g
    Max. capacity 48 × 1.5/2.0 mL
    Rotors available 9
    Acceleration time1 18 s
    Deceleration time1 18 s
    Display large LCD
    Timer 10 s to 9:59 h
    Noise level < 46 dB(A) with Rotor F-45-48-11
    Volume range 96 mL
    Power supply 120 V, 50 – 60 Hz
    Max. power consumption 550 W
    Dimensions (W × D × H) 31.9 × 54.0 × 25.4 cm
    Weight w/o accessories 30 kg
    Cooling refrigerated
    Temperature control range -11 °C to 40 °C

    1 Acceleration and deceleration times may vary depending on rotor, load and voltage version.


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    Eppendorf 5427 R Refrigerated Centrifuge Accessories

    • No sale
      Aerosol-tight, incl. rotor lid Aerosol-tight for safe centrifugation of hazardous samples Eppendorf QuickLock lid ...
        $1,207 USD
    • No sale
      Aerosol-tight with Eppendorf QuickLock® lid Perfect for phase-separation applications such as phenol-chloroform
        $1,712 USD
    • No sale
      Max. speed: 11,710 × g (10,500 rpm) PCR strip rotor for up to 48 × 0.2 mL PCR tubes
        $845 USD
    • No sale
      Incl. rotor lid Max. speed: 18,210 × g (12,700 rpm) Max. capacity: 48 × 1.5/2.0 mL tubes
        $935 USD
    • No sale
      Incl. rotor lid
        $761 USD
    • No sale
      Aerosol-tight, incl. rotor lid Aerosol-tight with Eppendorf QuickLock li
        $1,082 USD
    • No sale
      Rotor with higher edge for safe centrifugation of up to 24 spin columns Aerosol-tight with Eppendorf QuickLock lid
        $1,207 USD
    • No sale
      Aerosol-tight, incl. rotor lid Aerosol-tight with Eppendorf QuickLock lid
        $1,082 USD
    • No sale
      Aerosol-tight, incl. rotor lid, for 12 × Eppendorf Tubes 5.0 mL
        $1,353 USD